FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_385.352000000a0fda.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_385.352000000a0fda.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104547
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA3190.30512592422546797No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG3050.2917348178331277No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG2820.2697351430457115No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT2790.26686562024735283No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT2460.23530086946540793No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT2450.23434436186595503No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC2360.22573579347087916No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC2330.2228662706725205No Hit
GTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAA2220.21234468707853885No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG2190.2094751642801802No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAACTCACTC2150.20564913388236872No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA2120.20277961108401008No Hit
CTTCAAGGGACATCCTTTTACTTTCAGGCTCACTTTTGTTGTGAGTCTTT2120.20277961108401008No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG2100.2008665958851043No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT2050.19608405788783995No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA2020.19321453508948128No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG1990.19034501229112263No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAAATAGGAGTA1940.18556247429385825No Hit
CATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCCACTGATGAT1770.16930184510315935No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG1730.16547581470534783No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT1630.15591073871081906No Hit
TCCGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTT1610.1539977235119133No Hit
GTTTAGTCACTGGCAAACAGAGAAAAATGGCGAACAACAACATGACCACA1600.15304121591246042No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATC1580.15112820071355468No Hit
AGATAAAGGAGGAAGTAAACACTCAGAAAGAAGGGAAGTTCCGTTTGACA1500.14347613991793165No Hit
CCTATTTATGAATCCGTCTGCTGGAATTGAAGGGTTTGAGCCATACTGTA1470.140606617119573No Hit
TGACTACCCCGGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGT1460.13965010952012014No Hit
AAATACAATTGGACCGATTACCCTTCAACACCAGAGAGGTGCCTTGATGA1440.13773709432121437No Hit
TTATGAATCCGTCTGCTGGAATTGAAGGGTTTGAGCCATACTGTATGAAC1440.13773709432121437No Hit
GGATACAAGTCCTTATCAACTCTGCATAGATTGAATGCATATGACCAGAG1430.13678058672176152No Hit
GAGTGGAAGGCTTGTTGCTAAACTTGTTGCCACTGATGATCTTACAGTGG1430.13678058672176152No Hit
GTCCGGGAGCAACCAATGCCACCATAAACTTTGAAGCAGGAATTCTGGAG1410.13486757152285575No Hit
TTCCTCAAACACCCCAATGGATACAAGTCCTTATCAACTCTGCATAGATT1410.13486757152285575No Hit
TCCTTTTACTTTCAGGCTCACTTTTGTTGTGAGTCTTTATTCTTGACTCT1410.13486757152285575No Hit
GAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAAATGGCGAACA1390.13295455632394998No Hit
GATTGAATGCATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCC1360.13008503352559136No Hit
AATAGCAACTGTACGAAATACAATTGGACCGATTACCCTTCAACACCAGA1350.1291285259261385No Hit
CATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTCA1350.1291285259261385No Hit
GTTTAGGCGGTCTTGACCGGGGTAGTCAAGGGCTCTTTGCCATGAAAGCC1330.12721551072723275No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG1300.1243459879288741No Hit
CAATTGGACCGATTACCCTTCAACACCAGAGAGGTGCCTTGATGACATAG1290.12338948032942121No Hit
ACTCTGCATAGATTGAATGCATATGACCAGAGTGGAAGGCTTGTTGCTAA1270.12147646513051547No Hit
ATTCTGGAGTGCTATGAAAGGCTTTCATGGCAAAGAGCCCTTGACTACCC1270.12147646513051547No Hit
CCTTTATCTTTTGCCTTGCATCTTTGTTGTTCATGTCCCTTAATACTATT1260.12051995753106258No Hit
ATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTC1250.11956344993160971No Hit
GGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTT1240.11860694233215682No Hit
AGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAAATGGCG1230.11765043473270397No Hit
GAATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCG1230.11765043473270397No Hit
GCTATGAAAGGCTTTCATGGCAAAGAGCCCTTGACTACCCCGGTCAAGAC1230.11765043473270397No Hit
GCATAGATTGAATGCATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTG1210.1157374195337982No Hit
GTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACCAGTC1200.11478091193434531No Hit
GTTTCTAATAATGATAATGATACAACCATGTCAGCTATTATGGAGCTGTT1190.11382440433489244No Hit
TTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGGGAGTCTTATCCCAAT1170.1119113891359867No Hit
ACATAGAGGAAGAACCAGAGGATGTTGATGGCCCAACTGAAATAGTATTA1160.11095488153653381No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG1150.10999837393708092No Hit
CTTCTGGTGATAATCGGTGCTCTTGACCAAATTGGGATAAGACTCCCACC1140.10904186633762805No Hit
CTCTTCGAGCGTCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGA1140.10904186633762805No Hit
GGATTTGTTTAGTCACTGGCAAACAGAGAAAAATGGCGAACAACAACATG1130.10808535873817518No Hit
CCTTAATACTATTTCAGTTGGGCCATCAACATCCTCTGGTTCTTCCTCTA1110.10617234353926942No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA1110.10617234353926942No Hit
CCAGAGAGGTGCCTTGATGACATAGAGGAAGAACCAGAGGATGTTGATGG1110.10617234353926942No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA1110.10617234353926942No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC1110.10617234353926942No Hit
TTCATAAATAGGAGTACTTTCATCATTTTTACTCCAATTGCTTTTCTCTC1110.10617234353926942No Hit
CCTCTATGTCATCAAGGCACCTCTCTGGTGTTGAAGGGTAATCGGTCCAA1100.10521583593981654No Hit
CTATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCA1080.1033028207409108No Hit
ATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTG1080.1033028207409108No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC1080.1033028207409108No Hit
ACACCAGAGAGGTGCCTTGATGACATAGAGGAAGAACCAGAGGATGTTGA1070.10234631314145791No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC1060.10138980554200504No Hit
CTCTGGTGTTGAAGGGTAATCGGTCCAATTGTATTTCGTACAGTTGCTAT1050.10043329794255215No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAA100.0071211113144.732061
AGAAGCG350.003583737662.0280232
ATACCCT400.00608011954.274527
ACCCTCG450.00968569748.2440229
CTCTAGA450.00968569748.2440221
ATACCAG450.00968569748.2440228
CTAGAGG700.001061918741.3520163
TCTAGAG700.001061918741.3520162
GTAGAAA700.001061918741.3520161
CAGAAGC1102.4477605E-432.8936462
GTACAGA900.00364637432.162686
GCAGAAG1153.1771816E-431.4634911
GCACTGC950.00475094230.4699066
CAAGCAC1255.1784643E-428.946413
TTAAGGG3555.456968E-1226.5002354
ATACTAA1100.00972000326.3149191
ATTAAGG3751.0913936E-1125.086893
AGCAGAG1450.001232321624.9538047
GTATTAA4950.024.852981
TATTAAG4101.8189894E-1224.710352
Produced by FastQC (version 0.11.2)