FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_383.352000000a0fcd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_383.352000000a0fcd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138234
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTTGTGATTCTAATTCACTCACGTCATTAGCTCCCATTATGACTTCC2750.19893803261136914No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT1930.13961832834179724No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT1770.12804375189895395No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG1750.12659692984359855No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC1500.1085116541516559No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA1450.10489459901326736No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCGT100.007121421144.746736
ACTTACA259.4348076E-486.848058
GTAACAA1750.066.169944
GCAGAAG1009.094947E-1265.136041
CAGAAGC1154.0017767E-1156.640032
ACACGAG400.00608181654.280032
AGAAGCG400.00608181654.280032
CACGAGC400.00608181654.280033
ACGAGCA400.00608181654.280034
TAACAAG2350.049.2754865
ACAAGAG2500.046.318967
TAGTAAC2600.044.5374572
TAGATGT750.001491721438.5991336
GAGCAGA750.001491721438.5991331
TAGAAAC1001.3995722E-436.1866872
ATTACAA800.002048056836.1866846
TATAACA850.00275738634.0580562
AGCAGAG2404.3655746E-1133.1711273
ATCTGCG1102.4503737E-432.8969848
GCAGAGG2455.4569682E-1132.4941644
Produced by FastQC (version 0.11.2)