FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_380.352000000a0fb0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_380.352000000a0fb0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65171
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT810.12428841048932808No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA740.11354743674333675No Hit
CTATAGAACATGCTGGTGAATTCAAACATTCCAGTTTCGTTGCAGTAACT730.11201301192248087No Hit
GTAGTTGATTTCATTAACAAAGTATTTCCTTCTTTCACTCCCTACATGCT680.10434088781820135No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT670.10280646299734544No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCG100.0071135173144.742142
GGTGCGT100.0071135173144.742143
GGTGCAT203.8770324E-4108.55663
GCGGTGC352.7617716E-7103.387241
CGGTGCA259.413453E-486.8452762
AACACGA502.2631866E-886.8452766
ACACGAG554.3837645E-878.950267
GAAACAC554.3837645E-878.950264
AAACACG608.0124664E-872.371065
GTAGTAA1800.072.371061
GTAACAA1950.070.5153964
TAGTAAC2000.068.752512
TAACAAG2050.067.075625
CACGAGC702.329034E-762.0323458
AGCGGAG350.003576691562.0323451
CAAGAGG1200.060.3092238
AGTAACA2400.057.2937623
ACAAGAG2550.056.761627
AAGAGGA1405.456968E-1251.693629
TATATCC604.9541873E-448.247383
Produced by FastQC (version 0.11.2)