FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_379.352000000a0f97.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_379.352000000a0f97.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139110
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT2400.17252533965926245No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT2260.16246136151247215No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC2100.15095967220185466No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT1980.1423334052188915No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT1940.13945798289123715No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT1830.13155057149018762No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT1620.11645460427000215No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC1590.11429803752426138No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT1550.111422615196607No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT1530.10998490403277981No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA1520.10926604845086622No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC1500.10782833728703904No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC1420.1020774926317303No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA351.9135769E-9124.067882
GTAACAA1400.0113.728894
CTTGGGA203.8860098E-4108.5593955
GAGCAGA203.8860098E-4108.5593951
GATGGTT301.6085496E-596.4972467
GGTGCAT451.097942E-896.4972463
GTAATAG353.4568082E-582.711921
CAGAGCA801.8189894E-1281.419552
GAAACAC502.3198536E-672.372944
TAGATGG406.701039E-572.372935
TAGTAAC2200.069.083252
CATAACC651.4062061E-766.8057866
ACAAGAG2400.066.341867
AAACACG554.085581E-665.793575
AACCTCC451.2006388E-464.33159
GCGGAGC451.2006388E-464.33151
TAACAAG2500.063.6881835
GAGCATC1150.062.9329834
GCAGAGC1200.060.310781
GAAGCGG502.0216586E-457.898351
Produced by FastQC (version 0.11.2)