FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_377.352000000a0f8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_377.352000000a0f8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106640
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAACAACAGCTATGAGTGACTTGGCTAAGATTCACCCATGGGTCACTC1420.1331582895723931No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAC100.007119087144.747183
TTCGTTG100.007119087144.747187
CGCACTT501.9645086E-10101.323035
AGCACGC608.3127816E-1084.435851
GCACGCA702.8194336E-972.37362
ACGCACT808.105417E-963.3268934
AACACGA400.006077940654.2801936
ACACGAG450.00968222948.249067
CACGAGC450.00968222948.249068
ATAGTGC700.001061468641.356348
GTTGGGC700.001061468641.35634145
CACGCAC1354.9837035E-737.5270463
GTATTTA1051.8616505E-434.4636151
GGCCTAG850.002754527834.0581631
GTAGAAA2008.943971E-932.568121
AGTAGAA1801.293738E-732.1660421
GAGGATT1255.175986E-428.9494387
TAGTGCT1000.006102277428.9494389
TAGAGGC1100.00971590926.317674
TAGAAAC2756.7448127E-926.317672
Produced by FastQC (version 0.11.2)