FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_373.352000000a0f7d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_373.352000000a0f7d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285547
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT3390.118719510273265No Hit
GTGCTTGTGATTCTAATTCACTCACGTCATTAGCTCCCATTATGACTTCC3310.11591786991283397No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT3300.1155676648677801No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCACT502.2906534E-886.8474
CGCACTT502.2906534E-886.8475
GTATAGG554.096755E-665.793181
ACACGAG954.620233E-1060.945277
AAACACG1450.059.8944855
AGAAGCG1251.8189894E-1257.8980035
GCACGCA753.7975224E-757.8980032
CACGAGC1007.2759576E-1057.8980038
ACGAGCC502.0253447E-457.8980039
CACGCAC805.9303966E-754.2793773
TCTACCC400.00608872454.2793777
AGCACGC805.9303966E-754.2793771
GAAGCGG1501.0913936E-1148.2483336
ACGAGCA752.5725956E-548.2483339
GCTTAGC803.7668615E-545.2328151
TAGAAAC2900.042.425262
ACACGTG700.001064550441.3557137
AACACGT700.001064550441.3557136
GTATAAT1252.747529E-740.5286031
AACACGA1651.382432E-939.475916
Produced by FastQC (version 0.11.2)