FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_369.352000000a0f60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_369.352000000a0f60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137475
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG1530.11129296235679213No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC1430.10401891252955082No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC100.0071219564144.742811
AAACACG501.9645086E-10101.319975
ACACGAG554.420326E-878.950637
TGCAACT300.001945834672.3714142
CACGAGC502.3199973E-672.371418
GAAACAC851.31258275E-859.5999834
AACACGA753.7833524E-757.8971256
ACGAGCA400.006082398454.2785539
TAGAAAC1004.7291906E-850.6599852
GTAACAA1056.944538E-848.247614
TAGTAAC1056.944538E-848.247612
TGTATAC450.00968930748.2476042
TATAGGT657.378673E-444.536253
GTTCTAC907.527105E-540.2063371
GTATGCA1051.8647834E-434.4625781
CGATACC850.002757701334.057133145
GCTTAGC1153.1809806E-431.4658321
ACTCTCC1402.7629261E-531.0163178
ATGAGCA950.004754377530.4721729
TATACCG950.004754377530.4721727
Produced by FastQC (version 0.11.2)