FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_368.352000000a0f54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_368.352000000a0f54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118893
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGCTATTATCGTTTTGTGGAACAATATGTTATCATCAATTCTTTTAC1640.13793915537500104No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT1320.11102419822865937No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACGA501.9645086E-10101.324786
AAACACG703.092282E-1182.71415
ACACGAG651.5697879E-977.942147
GTAGTAC502.317407E-672.374843
AGTACAG554.081281E-665.795315
CACGAGC808.114512E-963.3279848
TAGTACA606.8385853E-660.312374
GGTAACA400.006079238454.2811284
GGTAGTA400.006079238454.2811287
TCTATAC400.006079238454.2811283
GAAACAC1101.6789272E-952.636254
TAGTAAC2150.050.4940762
TTAGTCA604.9695565E-448.2498979
ACGAGCA604.9695565E-448.2498979
GTATAGA450.00968428848.2498931
GAGATCC450.00968428848.2498938
GTAACAA2200.046.056724
AGTAACA2400.045.234283
CAGACCC803.7528E-545.2342769
GTAGTAA2500.043.4249041
Produced by FastQC (version 0.11.2)