FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_366.352000000a0f47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_366.352000000a0f47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110080
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTTGATTTCATTAACAAAGTATTTCCTTCTTTCACTCCCTACATGCT1920.1744186046511628No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACGA851.8189894E-1276.631696
ACACGAG903.6379788E-1272.3743747
ACGAGCC406.694161E-572.3743749
TCTATAC651.4032776E-766.807113
CACGAGC1009.094947E-1265.136948
AAACACG1051.6370905E-1162.035185
ACGAGCA553.2339804E-452.635919
AGTAGTA657.370744E-444.5380745
GAAACAC1601.0095391E-940.7105874
TTCTATA907.5154836E-540.207992
TAGTAAC1651.366061E-939.476932
TAGAAAC2151.2732926E-1137.0287482
TATACAG1406.628088E-736.187195
TATATCC1001.3976044E-436.1871873
TACAGCA1001.3976044E-436.1871877
TATTAGT1051.8619232E-434.463991
GTAACAA1905.4242264E-934.2825974
GTAGTAA1751.0106487E-733.085431
TAGTAGT1153.176124E-431.4671214
GTAGAAA2558.731149E-1131.2203181
Produced by FastQC (version 0.11.2)