FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_361.352000000a0f2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_361.352000000a0f2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123792
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACCTTACT7280.5880832363965361No Hit
GTATGCACTCTGTATCACCCAAGGGTTGTTACTAATACATTCTTCTATTC2550.20599069406746803No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC2330.1882189479126276No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT2230.18014088147860927No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG2000.16156132868036707No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT1590.12844125630089182No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT1580.12763344965748996No Hit
GTGCTTGTGATTCTAATTCACTTACATCATTGGCTCCCATTATGACCTCC1490.12036318986687346No Hit
ATGTTAATCCATCCCCTGTGGCCCACTTGGCATAATTGGTTTTCTGTAAT1460.11793976993666797No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG1410.11390073671965878No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACC1390.11228512343285511No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC1380.11147731678945327No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG1370.11066951014605145No Hit
ATATTGTCTATGTAGCTCCTCATTCCTTCGAAATTGGAGAAACCAGCTGG1360.10986170350264961No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT1350.10905389685924778No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA1320.10663047692904228No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC1280.10339925035543493No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAGA100.007121964144.736971
AAACACG502.2808308E-886.842185
GGTAGTA259.4349886E-486.842182
GAAACAC608.3309715E-1084.42994
ACGAGCA451.2398032E-680.4094249
GTAATGC554.084406E-665.7895361
ACACGAG606.843813E-660.307077
CACGAGC606.843813E-660.307078
TTATTCA2350.058.5106939
AACACGA651.0997877E-555.6680686
ATGCTAA400.006081941554.2763671
GCTGTCC400.006081941554.2763678
GTGTCCT2600.052.8846661
CATGTAC450.00968858848.2456553
CACGACA657.377274E-444.5344549
GCACGAC700.001062501541.3534168
TAGATGT700.001062501541.3534164
ATGCACG700.001062501541.3534166
TGCACGA700.001062501541.3534167
TTTATTC3400.040.441218
Produced by FastQC (version 0.11.2)