FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_348.352000000a0f10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_348.352000000a0f10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121974
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAAAGAATCTCTTAATTATTGCTGCTTTCAATATTCTCTCTGTAT1230.10084116287077574No Hit
CACTAGACAAATACTTTACTTTTATTTTTTTTATTTCAGTAGGTTTTTGG1220.1000213160181678No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAA100.0071190326144.756041
TAGTAAC2150.0100.992592
GTAGTAA2350.095.477391
CGCACTT554.413232E-878.957855
GTAACAA2750.078.957854
TAACAAG2900.074.873825
GTCTCGC502.3171651E-672.378031
AGTAACA3400.065.991733
TCTCGCT651.0988342E-555.6754042
AGCACGC651.0988342E-555.6754041
ACGCACT805.899001E-754.283524
AAGCGGT400.006078560454.283527
ACGAGCT400.006078560454.283524
AAGAGCG1258.913048E-1152.112189
CGCTCTG701.7040382E-551.6985865
TCTGTCG701.7040382E-551.6985868
CTCGCTC701.7040382E-551.6985863
ACAAGAG4400.050.9936077
CAAGAGC4200.049.9753048
GCACGCA752.5627001E-548.2520182
Produced by FastQC (version 0.11.2)