FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_347.352000000a0f03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_347.352000000a0f03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116974
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCCATTTTTCATCTGCAACACAGAAAGTTGGGGGGAGTT1990.1701232752577496No Hit
GTAGTAACAAGAGCGGCTAAAAATCTACTTATTTAACAAAAATCCCAAAT1690.144476550344521No Hit
ATACAGTGTCTGAGGAAGCCCAGATATTGGACACACTAGACAAATACTTT1620.13849231453143435No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA1500.1282336245661429No Hit
CACTAGACAAATACTTTACTTTTATTTTTTTTATTTCAGTAGGTTTTTGG1480.126523842905261No Hit
GAGTAACACCTGGGAAAGCTTCACCAGAGGGTCCTTTTCCACCACAGCAA1390.11882982543129242No Hit
GTATATAAAGAATCTCTTAATTATTGCTGCTTTCAATATTCTCTCTGTAT1220.10429668131379623No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGCG100.0071197557144.748619
GTAGTAA2350.0104.711761
TAGTAAC2350.0101.6322
GTAACAA2500.098.4290544
CGCACTT1000.094.086595
AGCACGC1000.086.849171
AAGAGCG608.3309715E-1084.436689
CTGTCGT353.4541743E-582.713491
CTCGCTC353.4541743E-582.713493
AGTAACA3200.079.159393
TAACAAG3300.074.567475
TCTCGCT406.6959496E-572.3743062
GTCGTCC406.6959496E-572.3743063
CGAGCTA406.6959496E-572.3743065
GCACGCA1200.072.37432
TCGCTCT451.19972916E-464.332724
TGTCGTC451.19972916E-464.332722
GTAGAAA2050.060.0177151
GTATAGA502.0201315E-457.8994451
ACGAGCC502.0201315E-457.8994452
Produced by FastQC (version 0.11.2)