FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_346.352000000a0ef5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_346.352000000a0ef5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149897
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT451.2414912E-680.405045
AGCACGC808.143616E-963.3189661
GTTACAC350.003587070262.026743
ACGCACT753.7881546E-757.891634
AACACGA502.0233267E-457.891636
GTAGTAA1550.056.0241551
TAACAAG1850.046.9391565
ACACGAG657.384268E-444.532027
AAGAGCG657.384268E-444.532029
GTAACAA1950.044.532024
GCACGCA1002.7537408E-643.418722
TAGTAAC2000.043.418722
GTATTAG907.534583E-540.202521
CACGAGC750.001493019438.5944188
AAACACG951.03605955E-438.0865975
AGTAACA2603.6379788E-1233.3990143
TGGACTG900.003651763832.1620145
CACGCAC1352.1575643E-532.1620143
GTAGAAA2257.9671736E-1032.1620141
CCTAAGG1402.7663744E-531.013372
Produced by FastQC (version 0.11.2)