FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_344.352000000a0ee8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_344.352000000a0ee8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37500
Sequences flagged as poor quality0
Sequence length151
%GC40

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTTGATTTCATTAACAAAGTATTTCCTTCTTTCACTCCCTACATGCT1620.432No Hit
GATTCATTTCTGTTTGAACCCAAGCAGCCACTCTACATTTGTTAGGGATT740.19733333333333333No Hit
GCCACTCTACATTTGTTAGGGATTTTAGGCTCTTCTTGGCACATTGTTTC560.14933333333333332No Hit
ATTGCTACTTCTTTCATTATTTTCTGGTATGTTAATCCATCCCCTGTGGC520.13866666666666666No Hit
GTTCTTTGGTTGTCCCCATCTCCCACATCTTTGGTGTATCATATGTTATC440.11733333333333333No Hit
ATATTGTCTATGTAGCTCCTCATTCCTTCGAAATTGGAGAAACCAGCTGG410.10933333333333334No Hit
AGATTCATTTCTGTTTGAACCCAAGCAGCCACTCTACATTTGTTAGGGAT400.10666666666666667No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA400.10666666666666667No Hit
GTGCTCAATAGATTCATTTCTGTTTGAACCCAAGCAGCCACTCTACATTT390.104No Hit
ATGTTAATCCATCCCCTGTGGCCCACTTGGCATAATTGGTTTTCTGTAAT380.10133333333333333No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCGG100.0071240165144.589326
AGAAGCG100.0071240165144.589325
ACGAGCT100.0071240165144.589324
AAACACG203.880322E-4108.441995
AGCATAT352.7560054E-7103.278091
GATTTCA502.3016419E-672.294667
ACGAGCA300.001942933472.294669
CATATAC554.0536215E-665.722423
CAGCATT554.0536215E-665.722429
GCATATA554.0536215E-665.722422
ACAGCAT451.1956565E-464.2619258
CACGAGC350.003579797561.9668543
AACACGA350.003579797561.9668541
ACACGAG400.006073515454.2209972
CTATAGA400.006073515454.2209971
TAGAAAC400.006073515454.2209972
TATACAG553.2239803E-452.5779385
TGATTTC701.6927954E-551.6390466
TAGAACA604.952829E-448.196444
TCCTAAT450.00967530448.196441
Produced by FastQC (version 0.11.2)