FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_340.352000000a0edb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_340.352000000a0edb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105896
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGT100.007118307144.752016
ACTCGCA100.007118307144.752019
TCGTGCA350.00358190962.036579
AACACGA350.00358190962.036576
AAGACGC400.006077021454.2820056
GAAGCGG553.2330558E-452.6370936
AGAAGCG553.2330558E-452.6370935
AAACACG553.2330558E-452.6370935
GCTACAC450.00968076848.2506682
TAAGACG450.00968076848.2506685
GTAGTAA1002.7430433E-643.4256021
TAGTAAC951.03303406E-438.0926322
CCTGATG1001.397059E-436.1880043
GACCATG850.002753991234.0592967
TAACATA1501.1363336E-633.7754674
ATATACT900.00364412532.1671144
GAGCAGA1402.7557739E-531.0182861
GATATAA1204.0744798E-430.1566681
CTAGACT1000.006101091428.9504034
GTGTATA1000.006101091428.9504039