FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_338.352000000a0ece.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_338.352000000a0ece.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109594
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG1280.11679471503914449No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA1260.11496979761665785No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT1210.11040750406044125No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG1180.10767012792671131No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA1180.10767012792671131No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG1110.1012829169480081No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTG100.0071263444144.699687
GGACCCC100.0071263444144.699688
CACGTGC100.0071263444144.699688
ACGAGCC100.0071263444144.699689
CCTGCGC100.0071263444144.69968145
AACACGT100.0071263444144.699686
AAACACG451.0983058E-896.466455
CACGAGC406.7052795E-572.349848
ACACGAG451.20139965E-464.310977
AACACGA451.20139965E-464.310976
GAAACAC702.3486609E-762.014154
ACCCAGC502.0229412E-457.8798754
TAGAAAC1251.8189894E-1257.879872
CTCAGGC553.2393332E-452.6180652
TCCTGTG701.7068187E-551.6784552
GTGTAAA701.7068187E-551.6784551
AATTTGC450.00969550548.2332277
CCTGTGT657.382915E-444.522983
GACCCAG657.382915E-444.522983
GTAACAA1351.0304575E-842.8739784