FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_337.352000000a0ea5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_337.352000000a0ea5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178037
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA3200.0106.2995451
TAGTAAC3450.098.596682
GTAACAA3700.091.934744
CGCACTT750.086.848985
AGTAACA4550.076.350763
TAACAAG4900.070.897135
AAGAGCG955.456968E-1268.5649959
CTCGCTC754.8912625E-967.549213
TCTGTCG1100.065.7946858
GTCTCGC954.6020432E-1060.9466631
GCACGCA1102.5465852E-1159.2152182
AAGCGGT502.0230349E-457.8993262
TCTCGCT1007.239578E-1057.8993262
AGCACGC1154.0017767E-1156.6406481
CGCTCTG1051.1168595E-955.1422165
CAAGAGC6100.053.3907748
GCGGTGC953.1628588E-853.3283271
ACGCACT1259.094947E-1152.109394
ACAAGAG6550.051.9326027
AACACGA1457.2759576E-1249.913216
Produced by FastQC (version 0.11.2)