FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_333.352000000a0e98.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_333.352000000a0e98.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149465
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC4470.29906667112701973No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA3490.23349948148395946No Hit
GTGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACCTTACT2990.2000468337068879No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA2670.1786371391295621No Hit
CTGTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTC2500.16726323888535777No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT2360.15789649750777773No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACT2340.15655839159669488No Hit
GTTAGCTATTATCGTTTTGTGGAACAATATGTTATCATCAATCCTTTTAC2110.14117017361924195No Hit
CCCATACACTGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA2060.1378249088415348No Hit
GTATTCAGGGTCCATTAGATTGTACTTCAACACTATTTCTGGGATATGCA2060.1378249088415348No Hit
ATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATA2010.13447964406382767No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG2000.13381059110828622No Hit
TCTCAGGCTTGTCCAATGAATCAATGATATCTTGGCAAAAGGGTACCAAT1910.12778911450841335No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC1860.12444384973070619No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT1820.12176763790854045No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT1790.11976047904191617No Hit
CCCCATATACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAG1780.11909142608637474No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT1750.11708426721975046No Hit
TTCTTAATCTATGGGCCATAGCTTCAAGCATGCTGTGCTGACCTACTGGT1660.11106279061987756No Hit
GTACAATGGTAGACCCAACAAATGGACCATTACCTGAAGACAATGAGCCA1650.11039373766433613No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA1640.10972468470879469No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT1510.10102699628675609No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAC100.007120225144.758793
ACCGTGC100.007120225144.75879145
TCAGGAG203.8852022E-4108.5690843
AAACACG700.093.059225
AACACGA750.086.855276
GAAACAC851.8189894E-1276.6374
AGAAGCG300.001945317172.3793955
AAGCGGT300.001945317172.3793957
CACGAGC851.70985E-1068.121788
ACACGAG902.8376235E-1064.337247
GACTATC451.2004E-464.337242
AGCGGTG350.00358413462.039488
GCGGAGC350.00358413462.039481
ACGAGCC502.0212571E-457.9035079
GTAACAA2350.055.4395334
ACTATCC553.2366317E-452.6395573
GCACAGG701.7057158E-551.6995662
CAGGATT701.7057158E-551.6995665
GTATAGT858.975876E-751.0913351
ACAAGAG2950.049.0707747
Produced by FastQC (version 0.11.2)