FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_328.352000000a0e8b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_328.352000000a0e8b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155290
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACCTTACT5580.3593277094468414No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG2390.15390559598171163No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC2100.13523085839397259No Hit
GCTTTGGTCATGTCATATCCTTGTATCGATGATTCTTGTTCAACAATTGC1990.12814733724000257No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG1850.11913194668040442No Hit
GTATGCACTCTGTATCACCCAAGGGTTGTTACTAATACATTCTTCTATTC1840.11848799021186168No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT1830.11784403374331895No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT1780.11462425140060532No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG1770.11398029493206259No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT1700.1094725996522635No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA1650.10625281730954987No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT1640.10560886084100714No Hit
GGGGCTATTTACTCTGTCTCCCTTGAAACAAGCTTTGGTCATGTCATATC1580.10174512202975078No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCT100.0071211536144.754278
CGCACCG100.0071211536144.75427145
ACACGAG301.1084376E-7120.6285557
CACGAGC301.1084376E-7120.6285558
AACACGA301.1084376E-7120.6285556
ACGAGCC203.8859714E-4108.5657049
GAAACAC406.1548417E-790.471424
AAACACG406.1548417E-790.471425
TAGTAGA300.001945700672.377142
TTATTCA2500.063.6918759
CAGGATT502.021814E-457.901715
TTTATTC3100.051.3644188
GTATGTA1301.3278623E-1050.1072461
GTGTCCT3350.049.691761
TTAGACC450.00968862548.2514238
CTAAATA450.00968862548.2514232
CAGAAGC1551.4551915E-1146.6949272
TATACAG657.3787634E-444.5397765
GTAACAA2700.042.8901524
TAGTAAC3000.041.013712
Produced by FastQC (version 0.11.2)