FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_327.352000000a0e7e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_327.352000000a0e7e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170153
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT3990.2344948369996415No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT1990.11695356532062322No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG1760.1034363190775361No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAG554.1836756E-1092.1156547
AACACGA554.1836756E-1092.1156546
GAAACAC502.284105E-886.851894
GAGCAGA259.436926E-486.851891
AGAAGCG353.4581688E-582.716094
GTAACAA1050.082.716094
AAACACG554.42451E-878.9562765
CACGAGC608.086499E-872.376588
TAGAAAC902.8376235E-1064.334732
CAGAAGC1051.6370905E-1162.0370643
CACAGGA350.0035855462.0370643
GAAGCGG502.0224453E-457.901265
TCTCGGG400.00608315654.282436145
CTTTAAG701.7071172E-551.6975569
AACCTCC604.9750664E-448.2510579
TAAATAC1056.9538146E-848.251054
ACGAGCA752.5673206E-548.251059
GACTCTA450.00969050748.25105145
GTGTAAA1101.0027543E-746.0578271
TAGTAAC2100.044.8045462
Produced by FastQC (version 0.11.2)