FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_325.352000000a0e61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_325.352000000a0e61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125903
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCTC100.0071194055144.75536145
CGACGAA100.0071194055144.75536145
ACGCACT350.00358315862.038014
CGCACTT350.00358315862.038015
AACACGA350.00358315862.038016
GCACGCA450.00968411348.251792
CACGAGC450.00968411348.251798
GTTATTA604.9697276E-448.251791
AGCCTAG800.00204676736.18884145
AAGACTG1501.1384036E-633.7762535
GTAGTAA1000.00610473728.9510751
GAATATA1050.007746877627.572451
TAACAAG1358.1230554E-426.806553
GTACACT1450.001232569324.9578233
TACACTG1801.5274111E-424.1258954
GAAGACT1550.0018175323.347644
TTATAAC1550.0018175323.347641
AGTAACA1550.0018175323.347641
GTGTACA1902.2034661E-422.8561081
GTAACAA1600.002186028322.6180252