FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_323.352000000a0e55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_323.352000000a0e55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137855
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCC100.007120001144.75627145
TAGTAAC1600.081.42542
GTAGTAA2100.062.03841
GTAACAA2200.055.928554
AAGAGCG651.099582E-555.675489
AGTAACA2550.051.0904433
TAACAAG2450.050.221565
GAGCCGC450.00968581248.2520876
GAGCGAG604.971232E-448.2520839
AAACACG1255.2168616E-946.3225
AACACGA1101.0007352E-746.0588076
AGAAGCG700.001062226741.3589325
GCGGTGC750.00149122738.601671
GTAGAAA2600.036.1890641
CAAGAGC3450.035.6645858
ACACGAG1458.7405897E-734.9411667
GTATATA1458.7405897E-734.9411661
CACGAGC1601.8862411E-631.6654328
AACAAGA4000.030.7607046
GAAGCGG950.00475226630.4750021