FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_320.352000000a0e3b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_320.352000000a0e3b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100030
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA100.0071188267144.744499
GTAACAA1750.078.5755844
AGCACGC300.001944188672.372251
AAACACG809.822543E-1172.3722465
TAGTAAC1750.066.1689152
ACGCACT451.1990499E-464.330894
GTAGTAA2250.061.1143461
TAACAAG2150.060.590725
CGAGCTA400.00607728954.2791863
CGCACTT400.00607728954.2791865
AACACGA953.1448508E-853.326926
AGTAACA2650.051.8895383
ACACGCC450.00968119848.2481656
GTGTAAA450.00968119848.2481651
CATAGAG657.368564E-444.5367662
GAAACAC1151.4150464E-744.052674
GTAGAAA1807.094059E-1140.2068061
ACAAGAG2750.039.4757737
GTCTAAT951.03293976E-438.0906561
TAGAAAC2002.237357E-1036.1861272