FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_318.352000000a0e2e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_318.352000000a0e2e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165777
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCG100.0071214708144.755057
ACGAGCC301.6089572E-596.5033659
CGCACTT801.0004442E-1072.3775255
ACGCACT1051.1168595E-955.1447834
GCACGCA953.1599484E-853.330812
GTAACAA2200.052.6382034
TAGTAAC2350.052.358212
AGCACGC1004.7341018E-850.664271
GTAGTAA2300.050.3495831
ACGAGCT450.00968956548.2516864
GTATTGC604.97437E-448.2516821
TAACAAG2650.046.4308665
AGTAACA2900.042.4282073
AACACGA1157.189672E-637.7621886
ACACGAG1209.626738E-636.1887637
AAACACG1209.626738E-636.1887635
TATTAGA1458.755378E-734.9408762
CACGAGC1251.27340645E-534.741218
CACGCAC1751.0155418E-733.086873
GTTGATT1102.4516482E-432.8988764
Produced by FastQC (version 0.11.2)