FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_316.352000000a0e11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_316.352000000a0e11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191582
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAATATATTGCTAAAATTCTCACCAACAGAAATAACTGCACCAACAA2920.15241515382447204No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT2230.11639924418786735No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT2150.1122234865488407No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA2120.1106575774342057No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC2030.10595985009030076No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT1950.10178409245127412No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACGC100.0071220463144.756973
GTAACAA2000.090.4731144
TAGTAGA259.4377616E-486.854193
GGTGCAT353.4588287E-582.718283
GCGGAGC651.5752448E-977.946071
GAAACAC1250.075.2736364
AAACACG1300.072.3784945
GAGCAGA406.704943E-572.378491
TAGAAAC1400.067.20862
TAACAAG2800.067.20865
CGGTGCA451.2013352E-464.336442
AACACGA1350.064.336436
TAGTAAC2850.063.4899022
ACAAGAG3100.063.039337
CGGAGCA702.3516077E-762.0387082
ACACGAG1650.057.0254757
AGAAGCG400.006083686454.2838673
CACGAGC1750.053.7668768
ACGAGCA1405.456968E-1251.6989259
GAGCATC1850.050.8605584
Produced by FastQC (version 0.11.2)