FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_310.352000000a0e04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_310.352000000a0e04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175908
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGCG100.0071221017144.753279
GTAGTAA1600.072.376631
CTCGGTT350.0035857362.037117145
CGCACTT1102.5465852E-1159.2172435
ACGCACT902.0641892E-856.2929344
TAGTAAC1950.055.6743352
TAATACG400.006083480554.2824754
AATACGG400.006083480554.2824755
GTATAGA1152.5011104E-950.348961
GCACGCA1101.00300895E-746.0578582
GTATACC1151.4226316E-744.055344
AACACGA1053.850939E-641.3580786
AGCACGC1252.7382703E-740.5309141
GTAACAA2800.038.7731974
TAACAAG3100.037.3556825
AAACACG1406.65661E-736.188325
TGTATAC2009.0094545E-932.5694853
AGTAACA3350.032.4074483
GTATTAC950.00475627830.4743731
CACGAGC1453.514858E-529.9489528