FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_306.352000000a0df6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_306.352000000a0df6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190404
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA3110.1633369046868763No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA3100.1628117056364362No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT3080.16176130753555598No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC2980.1565093170311548No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT2510.1318249616604693No Hit
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG2460.12919896640826875No Hit
CTATAGAACATGCTGGTGAATTCAAACATTCCAGTTTCGTTGCAGTAACT2400.12604777210562804No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG2200.11554379109682571No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT2160.11344299489506524No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT2110.11081699964286464No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT2060.10819100439066406No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC2040.10714060628978382No Hit
TCTCAGGCTTGTCCAATGAATCAATGATATCTTGGCAAAAGGGTACTAAT2020.10609020818890359No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC2010.10556500913846349No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG1940.10188861578538266No Hit
GTAGTTGATTTCATTAACAAAGTATTTCCTTCTTTCACTCCCTACATGCT1920.10083821768450242No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGT100.0071252044144.735292
ACGGAAA100.0071252044144.73529145
GAAACAC1100.0124.998664
AAACACG1250.0109.9988255
CGGTGCA203.8893672E-4108.551472
ACACGTG259.443293E-486.841187
ACACGAG1450.084.844837
AACACGA1450.084.844836
CACGAGC1600.072.3676458
GTAACAA3400.068.1107254
ACGAGCC451.2022078E-464.32689
ACGAGCA1400.062.029419
TAGTAAC3750.059.8239252
GCGGAGC502.0242945E-457.894121
ACAAGAG4100.056.482067
TAACAAG4150.055.8015565
CACGTGC400.006087237554.2757341
AACACGT400.006087237554.2757346
GTAGTAA4850.050.7319561
TAGAAAC3000.050.6573522
Produced by FastQC (version 0.11.2)