FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_302.352000000a0de9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_302.352000000a0de9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118471
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG300.001944689172.3759145
AAGCGGT300.001944689172.37597
TTCGGAT300.001944689172.37598
GAAGCGG406.695575E-572.375896
TAGTAAC1750.070.3080142
GTAGTAA1850.066.507581
GCGGTGC451.199662E-464.334139
CTTATGC400.006078838454.281923
CACGAGC553.234655E-452.6370128
AAACACG553.234655E-452.6370125
GTAACAA2350.052.357034
TAACAAG2450.050.220015
ACACGAG604.969131E-448.25067
AACACGA657.3722703E-444.5390136
AGAAGCG700.001061781541.3576555
CAAGAGC3100.039.6900068
AGTAACA3200.038.449693
GTAGAAA1752.4319888E-937.221891
AAGAGCG800.002046526236.1879469
ACAAGAG3500.035.1540077
Produced by FastQC (version 0.11.2)