FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_297.352000000a0ddc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_297.352000000a0ddc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166126
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA3520.2118873626042883No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT3280.19744049697217775No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC3130.18841120595210864No Hit
GTGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACCTTACT2800.1685467657079566No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT2330.14025498717840676No Hit
CTGTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTC2170.13062374342366637No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA2170.13062374342366637No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG2020.12159445240359726No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACT2010.120992499668926No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG1990.11978859419958345No Hit
CCCATACACTGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA1840.11075930317951434No Hit
CCCCATATACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAG1780.10714758677148671No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT1760.10594368130214415No Hit
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG1740.10473977583280161No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT1690.10173001215944524No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT1670.1005261066901027No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA600.0108.566682
GGTGCAT600.0108.566683
ACGCACT203.8863174E-4108.566674
AGAAGCG203.8863174E-4108.566675
CACGCAC203.8863174E-4108.566673
CGCACTT203.8863174E-4108.566675
GAGCGTT301.6089387E-596.5037164
AAACACG800.090.472235
AGCGTTT353.45764E-582.717475
AACACGA900.080.419766
GAAACAC950.076.187144
GCGGTGC1050.075.824351
CAGAGCG300.001945871572.3777852
GTAACAA2950.068.6975564
ACAAGAG3150.064.335817
ACACGAG1150.062.9372027
GCGGAGC350.003585152662.03811
AGTGTAG350.003585152662.03818
TAACAAG3300.061.4114535
GCGTTTT606.84889E-660.3148236
Produced by FastQC (version 0.11.2)