FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_286.352000000a0db2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_286.352000000a0db2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114484
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCTA406.695894E-572.3730859
CCTAGCT451.19971985E-464.3316358
TACCTAG651.0988482E-555.6716046
ACTGAGT450.00968430648.2487228
GTAACAA1151.4173384E-744.0531854
TAGTAAC1201.9800973E-742.2176362
TAACAAG1451.9319486E-839.929985
ACCTAGC750.00149067738.598987
TATACCT951.0338057E-438.0911034
GTAGTAA1354.988251E-737.5267871
ATACCTA800.002046626836.1865435
GGCACTT1051.8625829E-434.4633755
ATTAGTA850.00275546734.0579221
CTAACAC850.00275546734.0579223
GTGTATA1102.4478088E-432.896861
TTAAGGA900.003646073932.1658174
GTATAAA900.003646073932.1658171
GTAGAAA1902.0811967E-730.4728811
ATACCCT1255.1785534E-428.9492366
CTAATAT1753.7876598E-628.9492363