FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_285.352000000a0da6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_285.352000000a0da6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193737
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT3690.1904643924495579No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT3560.1837542648022835No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT3130.1615592271997605No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT2950.15226828122661135No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC2810.14504198991416198No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC2740.1414288442579373No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT2640.13626720760618777No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT2600.13420255294548797No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT2530.13058940728926327No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG2490.12852475262856347No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA2460.1269762616330386No Hit
CCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT2360.12181462498128906No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC2220.1145883336688397No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT2210.11407217000366478No Hit
GTGGAATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTT2140.1104590243474401No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAATC2140.1104590243474401No Hit
GCAAACAAGCACTACAATAAAGGAAAATACAGGGCTTAACTTCCCTTATA2020.10426506036534064No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACGG100.0071230396144.75064145
GTAACAA3000.0118.213034
AACACGA950.0106.658386
GAAACAC1050.0103.393314
AAACACG1050.0103.393315
TAGAAAC1100.098.693632
TAACAAG3650.097.161395
ACGAGCC502.2861059E-886.8503959
TCTTAAC353.459678E-582.714652
CGGTGCA353.459678E-582.714652
ACACGAG1250.081.060367
TAGTAAC4400.080.599792
ACAAGAG4700.075.4551247
GTAGAAA1650.074.568511
AGAAGCG502.3228476E-672.375333
CTTAACA406.706586E-572.375323
CAGCATC406.706586E-572.375325
AACCTCC300.001946624772.375329
AGTAACA5200.068.199823
CACGAGC1550.065.371268
Produced by FastQC (version 0.11.2)