FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_284.352000000a0d99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_284.352000000a0d99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171996
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT2510.14593362636340382No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA2090.12151445382450755No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC2040.11860740947463894No Hit
CTATAGAACATGCTGGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT1920.1116305030349543No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC1900.11046768529500686No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG1840.10697923207516455No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCG100.0071212985144.757789
GAGCTCC350.00358515162.0390479
TAGGGTG350.00358515162.0390475
GACCCCA400.006082497554.284179
CACGAGC1004.7346475E-850.6652228
ACACGAG901.332397E-648.2525947
ACGAGCC450.00968945548.2525949
AACACGA951.9336894E-645.712986
ACTGTCC1505.4023985E-1043.427338
GTGTAAA1557.4396667E-1042.026451
AAACACG1053.849611E-641.3593645
TATAGAG1406.653827E-736.1894455
GAAACAC1209.627594E-636.189444
TACATAC1707.8734956E-834.0606583
GCTGTCC850.002758220534.0606588
GCTGTAC1102.451757E-432.8994941
CTGTCCA2051.1455995E-831.7760969
TAGATGT950.004755261430.475324
GTACACT1204.0840323E-430.157874
GTACTAC1952.6242742E-729.6939011
Produced by FastQC (version 0.11.2)