FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_282.352000000a0d7f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_282.352000000a0d7f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209620
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGCATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA4460.2127659574468085No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT4410.21038068886556627No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT3200.15265718919950386No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTT3190.1521801354832554No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT3110.14836370575326782No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA2680.1278503959545845No Hit
GTTGTATTCAGTGCCGTTGCAGCAGGTTGGTTTCTGCATACTGTCCAATC2670.12737334223833602No Hit
ATCTATCACAGTCTTGTTTGCAATTTCTACAGCTAGCTCCAGTGGGTTTG2620.12498807365709377No Hit
GTATGCAGAAACCAACCTGCTGCAACGGCACTGAATACAACAATAACCTC2590.12355691250834844No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC2500.11926342906211239No Hit
GTGTAGCATTTCTATGAACTCAGCTGATGTTGCTCCTGCCACTGGCAAGA2440.11640110676462169No Hit
ATTAGTAGCAGATGGTGGGCCCAACATTTACAATTTGAGAAACTTGCATA2420.1154469993321248No Hit
GTACTGACCAGCCATTTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG2390.11401583818337944No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT2320.1106764621696403No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC2220.1059059250071558No Hit
TTACTGGACCATGTGCTGGAGTTATGTCTGCCTCTTTGATGCCCTCAATA2140.10208949527716821No Hit
AGATTATACTTTAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTA2120.10113538784467131No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAGA301.1134034E-7120.563373
AGAAGCG352.791021E-7103.3400345
GAGCAGA502.2952008E-886.805631
GTAACAA1150.081.7734154
GAAGCGG451.2459386E-680.375586
ACACGAG702.3618122E-762.004027
CACGAGC702.3618122E-762.004028
AAACACG702.3618122E-762.004025
TAGTAAC1550.060.67062
TAGAAAC1102.5465852E-1159.185662
CAGAAGC753.8051257E-757.870424
TAGATGT753.8051257E-757.870424
AACACGA753.8051257E-757.870426
GAAACAC805.9422746E-754.253524
GCCGTTT400.006097958454.25352145
GTGCCCG805.9422746E-754.253529
GGCCGTC1056.9934686E-848.2253467
GGGCCGT1056.9934686E-848.2253466
TGGGCCG1056.9934686E-848.2253465
ACAAGAG2100.044.780687
Produced by FastQC (version 0.11.2)