FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_280.352000000a0d62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_280.352000000a0d62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247382
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC3380.1366307977136574No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT3360.13582233145499673No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC3340.13501386519633604No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA3080.12450380383374698No Hit
GTTAGCTATTATCGTTTTGTGGAACAATATGTTATCATCAATTCTTTTAC2940.11884454002312214No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG2930.1184403068937918No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG2900.11722760750580075No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA2820.11399374247115797No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG2630.10631331301388137No Hit
ATATTTGCTTTCTCCCCTGGTTTTAGCTTTTCAAGTTCTTCAATGGTTTT2610.10550484675522066No Hit
ATATTGTCTATGTAGCTCCTCATTCCTTCGAAATTGGAGAAACCAGCTGG2600.10510061362589032No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT2580.10429214736722964No Hit
GTATGTAGAAACCAGCCTGCTGCAACAGCACTAAACACAACAATAACCTC2560.10348368110856894No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT2500.10105828233258685No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTC100.00712522144.743882
CGTGAAA100.00712522144.743888
TTTGCGT100.00712522144.743884
ACGAGCC406.168357E-790.464939
AACACGA1050.068.925666
GCGGAGC651.4108628E-766.804871
AAACACG1100.065.792675
ACACGAG1150.062.932127
AAGCGGT851.3182216E-859.600427
AGCGGTG851.3182216E-859.600428
GAAACAC1251.8189894E-1257.8975524
AGAAGCG902.069828E-856.289295
CACGAGC1301.8189894E-1255.6707238
TGACACC400.00608795354.2789576
GAAGCGG1004.749745E-850.6603626
GCGGTGC1004.749745E-850.6603629
AGCGGAG450.00969812148.2479633
GAGCAGA1351.0366421E-842.8870741
TAGTACA1903.6379788E-1241.8995444
AGCAGAA1451.9479558E-839.9293482
Produced by FastQC (version 0.11.2)