FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_279.352000000a0d56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_279.352000000a0d56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227097
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT4420.19463048829354856No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT3990.17569584802969657No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA3610.1589629101221064No Hit
GTATGTAGAAACCAGCCTGCTGCAACAGCACTAAACACAACAATAACCTC3260.1435509936282734No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC3100.13650554608823542No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG2740.12065328912315003No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT2730.12021294865189765No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG2710.1193322677093929No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG2650.11669022488187866No Hit
GCCCAAGCCATTGTTGCGAAAAATCCGCTTTTACTGGTAGCGTTAGGGCA2580.11360784158311206No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA2480.10920443687058834No Hit
TCTCAGGCTTGTCCAATGAATCAATGATATCTTGGCAAAAGGGTACCAAT2460.1083237559280836No Hit
GTAGTTGATTTCATTAACAAAGTATTTCCTTCTTTCACTCCCTACATGCT2340.10303967027305513No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGAGC353.4617406E-582.710921
ATAACAC300.001947406972.3720555
CAGAAGC1150.069.225442
GAAACAC808.1618055E-963.3255464
AAACACG808.1618055E-963.3255465
AGCGTTT350.00358797362.0331886
AATTGCG350.00358797362.0331885
GCTGTCC851.317494E-859.6005138
GAAGCGG805.9252125E-754.279041
GCGTTTT400.006087274354.279047
TTGCGAT400.006087274354.279047
CGGTGCG400.006087274354.279048
CGGTGCA400.006087274354.279042
ACGAGCC400.006087274354.279049
AACACGA805.9252125E-754.279046
AAGCGGT752.5710102E-548.248042
GTATGTA2100.048.248041
ACACGAG901.3351728E-648.2480357
GCGATTT450.00969704448.2480359
GGTGCGT450.00969704448.2480359
Produced by FastQC (version 0.11.2)