FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_274.352000000a0d49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_274.352000000a0d49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220608
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT3250.14732013344937628No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA2540.11513635044966637No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG2490.11286988685813751No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC2470.11196330142152597No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA2380.10788366695677401No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC2300.10425732521032781No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC2290.10380403249202205No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGTG256.5090226E-6115.800727
GCACAGG700.0103.393512
CGGTGCA501.9645086E-10101.325632
GAGCAGA607.2759576E-1296.500611
GTAACAA1650.083.341434
CAGGATT851.8189894E-1276.6328355
CACAGGA903.6379788E-1272.375463
AGAAGCG808.1581675E-963.328525
AAACACG702.3539542E-762.0361065
GCAGAAG1102.5465852E-1159.216281
AACACGA753.7924656E-757.9003646
ACAAGAG2600.055.6734247
ACACGAG805.9225204E-754.281597
GAAACAC805.9225204E-754.281594
TAGTAGA400.006085915554.281594
GAAGCGG1004.744652E-850.6628156
CAGAAGC1301.3278623E-1050.1060832
CACGAGC901.3345671E-648.2503058
TAGTAAC3000.048.2503052
TAGAAAC951.9368308E-645.710812
Produced by FastQC (version 0.11.2)