FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_264.352000000a0d3c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_264.352000000a0d3c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135905
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT1550.11405025569331517No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC1550.11405025569331517No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT1500.11037121518707922No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT1430.10522055847834884No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGTG151.2364984E-4144.739888
ACGAACG100.0071223144.73988145
AACACGC100.0071223144.739882
CGGTGCA256.502418E-6115.791912
AAGCGGT203.886454E-4108.554917
GAAGCGG300.001945943772.369946
GCGGTGC406.701857E-572.369941
AGAAGCG300.001945943772.369945
GCGGAGC406.701857E-572.369941
AAACACG606.846605E-660.3082855
AACACGA606.846605E-660.3082856
GAAACAC651.1002356E-555.6691864
GTAACAA1950.055.6691864
GGTGCAT400.006082738354.2774543
CAGCCTT553.2376708E-452.6326875
ACACGAG907.5275544E-540.2055247
TAACAAG2750.039.474515
TACCTTA1303.7190148E-738.968437
TAGTAAC2600.038.968432
CCATGTA750.001491981838.59734
Produced by FastQC (version 0.11.2)