FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_249.352000000a0d2f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_249.352000000a0d2f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207349
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT2710.13069751964079884No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT2300.11092409416008758No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT2260.10899497947904258No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC2230.10754814346825882No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC2160.10417219277643007No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG2140.10320763543590758No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC2130.10272535676564631No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT2090.10079624208460132No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCATC203.8888163E-4108.558794
CGGAGCA203.8888163E-4108.558792
GAGCAGA451.1006705E-896.4967041
GCGGAGC451.1006705E-896.4967041
AGCGTTT259.441956E-486.847036
GAGCGTT259.441956E-486.847035
GTAACAA1850.086.064634
GAGCATC554.4308763E-878.951846
GGAGCAT300.001947116372.372533
GAAACAC608.098323E-872.372534
AGCATCT954.6020432E-1060.9452865
AGAAGCG606.8578265E-660.310445
TAGTAAC2950.058.879342
GGTACTG651.1020338E-555.6711771
TGATTAA651.1020338E-555.6711776
AAACACG805.9224294E-754.2793965
TAACAAG2950.053.972735
GAAGCGG752.5701298E-548.2483526
ATCACGT450.00969561448.2483523
ACGTCCC450.00969561448.2483526
Produced by FastQC (version 0.11.2)