FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_137.352000000a0d12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_137.352000000a0d12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213847
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA4590.21463943847704198No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT2960.13841671849499876No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA2730.12766136536869818No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT2520.11784126034033679No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA2520.11784126034033679No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAACCTCATTTAGGTTGAA2490.11643838819342801No Hit
TCATTAAGACGCTCGAAGAGTGAATTGAGGATCCGATGGCCATCTTCTTC2490.11643838819342801No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC2350.10989165150785375No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG2320.10848877936094498No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC2210.10334491482227948No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG2210.10334491482227948No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTGT100.007123716144.749538
CGCACTT256.508866E-6115.799625
CACGCAC203.8885057E-4108.562153
GCACGCA203.8885057E-4108.562152
AGCACGC259.441203E-486.849721
ACGCACT300.001946961372.374774
AAACACG702.3537541E-762.0355155
ATAGTAG350.00358715462.0355153
ACTTAAG450.00969484148.249844145
GCGGAGC604.9785676E-448.2498441
AACACGA657.3862297E-444.5383156
TAACAAG3350.043.2088175
CAAGAGG2005.456968E-1239.806128
GTAACAA3650.039.6574064
TAGTAAC3700.039.1214942
GAAACAC951.03668106E-438.091984
AGTAACA4200.034.4641763
ACAAGAG4650.034.2418257
GTAGTAA4800.033.171771
CTATAGA1601.893377E-631.663961
Produced by FastQC (version 0.11.2)