FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_100.352000000a0d05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_100.352000000a0d05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179768
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA2550.0105.0138551
CGCACTT1250.098.42925
GTCTCGC800.090.468011
GTAACAA2950.088.321314
ACGCACT1500.086.84934
TAGTAAC3150.085.011212
CTCGCTC900.080.4160163
TCGCTCT950.076.1835864
TAACAAG3450.075.5211265
AGCACGC1750.074.4422451
GCACGCA1850.070.418342
TCTCGCT1050.068.9282
GCATACC554.0899467E-665.794923
TTAGGTC606.8530717E-660.3120083
CTGTCGT1102.5465852E-1159.2154279
TCTGTCG1206.002665E-1154.2808078
AGTAACA4800.054.2808073
GAATCTA553.239502E-452.6359376
CGGTGCA701.7078091E-551.6960072
GTAGAAA2100.051.6960031
Produced by FastQC (version 0.11.2)