FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_pr8_d4.352000000a0eb1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_pr8_d4.352000000a0eb1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124214
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG7280.5860853043940297No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA4010.3228299547555026No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG3360.27050090972032137No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT2580.2077060556781039No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC2260.18194406427616855No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT2220.17872381535092663No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA2040.16423269518733796No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT2010.16181750849340654No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG1970.15859725956816462No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1870.15054663725505982No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT1750.14088589047933406No Hit
CCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGTG1750.14088589047933406No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1680.13525045486016068No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT1650.13283526816622926No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT1630.13122514370360827No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1630.13122514370360827No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA1620.1304200814722978No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC1430.11512389907739869No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA1430.11512389907739869No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT1410.11351377461477773No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA1410.11351377461477773No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC1360.10948846345822533No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1360.10948846345822533No Hit
GTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTT1350.10868340122691485No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1320.10626821453298341No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACA1310.10546315230167291No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT1310.10546315230167291No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC1290.10385302783905195No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTT100.007121854144.73793145
CGAATTA100.007121854144.73793145
CTATTCG100.007121854144.737939
GGTTAAC151.2363103E-4144.737931
GAGCGAA650.0122.470551
ACAGAGT203.8858657E-4108.553456
GCAGGGG650.0100.203189
GCAAAAG3300.089.912963
GTTAACA259.434825E-486.842752
AGCAAAA3950.075.117152
AGCGAAA1350.069.688631
AGCAGGG3400.065.983478
GCAGGGT2500.063.684699
CAGAGTG350.003584753362.0305447
AAGCAGG5900.061.3296287
AGCAGGT2450.059.0767028
GAGCAAA5300.055.9835361
CTATCAA400.006081834454.2767262
AGAGTGT400.006081834454.2767268
CAAGGTA400.006081834454.2767269
Produced by FastQC (version 0.11.2)