FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_pr8-vww_5-1.352000000a11af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_pr8-vww_5-1.352000000a11af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84595
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG3650.43146758082629No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2180.2576984455346061No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG2020.2387847981559194No Hit
CCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGTG1370.16194810568000473No Hit
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA1100.13003132572847095No Hit
CCAAGAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGA1070.1264850168449672No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1070.1264850168449672No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1050.12412081092263137No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA1030.12175660500029553No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG1030.12175660500029553No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1020.12057450203912762No Hit
GCAGTTAAACTGTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGC940.11111767834978427No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT930.10993557538861636No Hit
ACCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGT930.10993557538861636No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG910.10757136946628051No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT900.1063892665051126No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCC900.1063892665051126No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC900.1063892665051126No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA850.10047875169927302No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGCT100.007116456144.747487
CAGACGA100.007116456144.747489
GAGCGAA301.10434485E-7120.62291
GCAAAAG1750.086.8484953
TCGCCAT300.001942986272.37374145
TCGTACT300.001942986272.373742
CTATTAA300.001942986272.373741
CAAAAGC2250.067.548834
GCAGGGG651.3994213E-766.806539
TCCGACT451.1978591E-464.3322148
GCAGGGT902.8194336E-1064.3322149
CCGACTA451.1978591E-464.3322149
AGCAGGG1700.063.859188
CTAAGCA350.003579857862.0346374
GAGCGGT350.003579857862.0346378
AGAGCGG350.003579857862.0346377
AGCAAAA2700.058.97122
GAGCAAA2850.055.8674551
GATAAGT400.00607355754.2803083
TAAGTTG400.00607355754.2803085
Produced by FastQC (version 0.11.2)