FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_pr8-vww_100-1.352000000a1185.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_pr8-vww_100-1.352000000a1185.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115481
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG6240.5403486287787602No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA4180.36196430581654127No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG3260.28229752080428816No Hit
CCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGTG2240.1939713026385293No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT2210.19137347269247754No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1950.16885894649336253No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1930.16712705986266138No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1890.1636632866012591No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG1800.1558697967631039No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA1750.15154008018635098No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT1680.14547847697889696No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT1660.1437465903481958No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1570.13595310051004061No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA1540.13335527056398888No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1450.12556178072583368No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT1440.12469583741048311No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1430.12382989409513254No Hit
GCAGTTAAACTGTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGC1360.1177682908876785No Hit
GTATTCATCACCCGCCTAACAGTAAGGAACAACAGAATCTCTATCAGAAT1360.1177682908876785No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA1320.11430451762627619No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT1300.11257263099557503No Hit
CCAAGAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGA1300.11257263099557503No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCC1260.10910885773417273No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT1260.10910885773417273No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC1240.10737697110347157No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC1230.106511027788121No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1210.10477914115741983No Hit
GCTTTCCACTAGAGGAGTTCAAATTGCTTCCAATGAAAATATGGAGACTA1180.10218131121136811No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTTAA151.2356277E-4144.75141
GAGCGAA450.0128.667921
TTTAAAC256.495664E-6115.8011253
TTTTAAA256.495664E-6115.8011252
TAAACTA352.7743954E-7103.393875
GCAAAAG3500.099.25813
CTATTCG301.6070788E-596.500949
GCAGGGT2500.086.8508459
AGCAAAA4500.078.8091052
GCAGGGG950.076.184959
AGCAGGG4000.074.18518
CAAAAGC4750.073.137564
GTATTGG406.694943E-572.37571
CCTCTAA300.001944584472.37578
GAGCAAA5300.066.913381
AGGGTGT651.403696E-766.808346
AAGCAGG6150.065.903087
CAAGGGT902.8194336E-1064.333964
GGACAGT451.1995495E-464.333966
AGCGAAA954.5656634E-1060.9479641
Produced by FastQC (version 0.11.2)