FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_pr8-vww_10-1.352000000a1192.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_pr8-vww_10-1.352000000a1192.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104419
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG5410.5181049425870771No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2870.2748541932023865No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG2580.24708146984744156No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1810.1733401009394842No Hit
CCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGTG1810.1733401009394842No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1750.16759402024535763No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1560.14939809804729026No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT1540.14748273781591473No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA1530.14652505770022697No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1460.13982129689041267No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG1440.13790593665903716No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1360.13024449573353508No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1340.12832913550215957No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT1320.12641377527078404No Hit
CCAAGAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGA1300.12449841503940853No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC1290.12354073492372078No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1270.12162537469234526No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT1220.11683697411390646No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT1190.1139639337668432No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT1170.11204857353546767No Hit
GTATTCATCACCCGCCTAACAGTAAGGAACAACAGAATCTCTATCAGAAT1150.11013321330409216No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA1140.10917553318840441No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT1110.10630249284134113No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA306.530172E-10144.745221
TCTACTA100.0071191685144.745215
GCAAAAG2150.0107.717363
GCAGGGT1450.089.841859
GCAGGGG703.092282E-1182.711559
AGCAGGG2500.081.057328
AGCGAAA554.4092303E-878.9519352
CAAAAGC3150.073.521384
AGCAAAA3250.073.486032
AAGCAGG3850.073.312517
AGGGTGT702.8194336E-972.3726046
AAGGGTG702.8194336E-972.3726045
GAGCAAA3850.069.5528951
TGTAATA350.003582437362.033662
GGACAGT651.0982185E-555.6712386
CAAGGGT1051.1095835E-955.141034
TACTATT657.369882E-444.5369874
AAAGCAG6350.043.3095936
TCGTACT855.363933E-542.572122
AAAAGCA5600.041.3557745
Produced by FastQC (version 0.11.2)