FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_pr8-vww_1-5.352000000a11c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_pr8-vww_1-5.352000000a11c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130896
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA3680.2811392250336145No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC2420.18487959907101822No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG2180.16654443222099988No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT2050.1566128835105733No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT1890.1443894389438944No Hit
GTGTAAATACAATAAGTAATGAGGAAACAGGTAACGAATTACAGAAAACC1420.10848307052927514No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC1330.10160738296051827No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGAG100.0071187555144.761952
TCTACTA151.2356437E-4144.761955
GGTGCAG100.0071187555144.761953
GCGGAGC100.0071187555144.761953
GTAACAA1950.0103.931654
CTATGCT301.6073043E-596.5079659
TCTATGC301.6073043E-596.5079658
CGGTGCA451.0966687E-896.507962
GAGCATC651.6370905E-1189.0842744
AGGATTG651.5697879E-977.948745
TAACAAG2800.069.795945
GCAGAGC1050.068.934261
TAGCACC955.456968E-1268.571454
AGCATCT955.456968E-1268.571455
AGCAGGG651.4044599E-766.81328
TATAGCA1009.094947E-1265.1428762
AGCACCG902.8376235E-1064.338645
ATAGCAC1150.062.9399723
ACAAGAG3050.061.7018057
CACCGGT954.5838533E-1060.9523967
Produced by FastQC (version 0.11.2)