FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_pr8-vww_1-100.352000000a11e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_pr8-vww_1-100.352000000a11e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130864
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA4090.31253820760484163No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC2540.19409463259567183No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG2330.17804743856217142No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT2070.15817948404450421No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT2000.15283041936667074No Hit
GTGTAAATACAATAAGTAATGAGGAAACAGGTAACGAATTACAGAAAACC1740.13296246484900354No Hit
GTATTTACACAGTCCCTCCACAACTTCCATAAGAAGTTTTCGTTAGCTTT1510.11538696662183642No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT1350.10316053307250275No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC1340.10239638097566939No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAACAAAAACTGAAAATCAA1310.10010392468516933No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCGG100.007119542144.756598
CACGCAC100.007119542144.756593
ACGTGCA151.2358258E-4144.756592
CGGTGCA351.9117579E-9124.077092
AGGATTG700.0124.077095
GAGCAGA256.4977758E-6115.805281
CACGTGC203.8843433E-4108.5674441
GAAACAC800.099.520164
GAGCATC554.1836756E-1092.117834
TAGAAAC950.091.425212
AAACACG900.088.462365
GCGGAGC259.4311277E-486.853961
AGCATCT608.3309715E-1084.4413455
AACACGA950.083.806446
CAGCCTT353.4547687E-582.7180565
GTAACAA1250.081.06374
GGATTGA1150.075.525186
ACAACAG502.318062E-672.37831
ACAGCCT406.697095E-572.3782964
GGTGCAT702.8248905E-972.3782963
Produced by FastQC (version 0.11.2)