FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_pr8-vww_1-10.352000000a11d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_pr8-vww_1-10.352000000a11d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96020
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA3610.3759633409706311No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG3170.3301395542595293No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT1930.2009997917100604No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC1580.16454905228077485No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT1540.1603832534888565No Hit
GTGTAAATACAATAAGTAATGAGGAAACAGGTAACGAATTACAGAAAACC1140.118725265569673No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT1110.11560091647573423No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC1100.11455946677775464No Hit
GTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGGG1060.11039366798583627No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA980.10206207040199958No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA151.234961E-4144.752091
GTAACAA1050.0103.394344
AGGATTG406.1373794E-790.4700555
GCGAAAG259.424582E-486.851263
GAAGCGG502.3137763E-672.3760451
GGATTGA502.3137763E-672.3760456
ACAAGAG1500.072.376047
TAACAAG1600.067.852545
AAACACG754.8585207E-967.550975
CTACAAG451.1984956E-464.33426145
ACACGAG702.3397115E-762.0366067
GAAACAC702.3397115E-762.0366064
AACACGA702.3397115E-762.0366066
CGGTGCA606.8279223E-660.313372
ATTCTGC1600.054.282032145
TAGAAAC901.3265562E-648.250692
CTCCTTG450.00967802548.250698
GCCACTC901.3265562E-648.250691
GTAAATA901.3265562E-648.250693
CACTCTA951.9252257E-645.7111853
Produced by FastQC (version 0.11.2)