FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_pr8-vww_1-1.352000000a11bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_pr8-vww_1-1.352000000a11bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78846
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA1820.23082971869213403No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1580.20039063490855594No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1010.1280978109225579No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG980.12429292544961063No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT860.10907338355782158No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG820.10400020292722523No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGAG100.0071156393144.746836
TCGTACT100.0071156393144.746832
CTTTAAG202.1368232E-6144.746839
TTCGGAT406.12843E-790.46677145
GTAACAA800.090.466774
CAGGGTG259.419626E-486.84819
AGGATTG554.3948603E-878.952815
TTGAGCG406.6828055E-572.373419
AGTAGAG300.001942528672.3734052
TAACAAG1100.065.7940145
GCCTTTA451.1973811E-464.3319247
GCAAAAG1051.6370905E-1162.034363
AGCGAAA350.003579018462.034351
GCAGAGC651.095659E-555.6718561
GGATTGA805.874663E-754.280066
CAAAAGC1056.892515E-848.2489434
GAATGAG450.00967301748.2489431
CACGAGC450.00967301748.2489431
GTAGTAA1751.8189894E-1245.491861
GCAGGGT803.7416456E-545.2333838