FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_vww_10e3_p2.351000000a117b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_vww_10e3_p2.351000000a117b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153975
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT4200.2727715538236727No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA2020.1311901282675759No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC1990.12924176002597823No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC1990.12924176002597823No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC1800.11690209449585973No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGCATCGCATGTAT1740.1130053580126644No Hit
GGATACAACTTAAAACGAATGCTGAAGACATAGGAACCAAAGGCCAAATG1680.10910862152946907No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT1680.10910862152946907No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT1610.1045624289657412No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGCATCGCATGTAT1580.10261406072414353No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTC151.2280006E-4145.03
CGCACTT301.0972326E-7120.8333365
GTAACAA1450.0110.04
TCTCGCT203.8597788E-4108.752
TAGTAAC1550.0102.903232
GCACGCA406.0927596E-790.6252
AAACACG900.088.6111155
CGCTCTG259.3715603E-487.05
CGGTGCA502.2559107E-887.02
GAAACAC950.076.315794
ACGCACT502.2969707E-672.54
GTCTCGC300.001932622372.51
AGCACGC502.2969707E-672.51
TAGAAAC1400.067.321432
TAACAAG2400.066.4583365
CACGCAC554.045316E-665.9090963
GAAGCGG702.321467E-762.1428571
AACACGA1300.061.3461536
CGAGCCT502.0048818E-458.05
ACAAGAG2850.055.9649167
Produced by FastQC (version 0.11.2)