FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_vww_10e3_p1.351000000a1046.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_vww_10e3_p1.351000000a1046.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127582
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA5660.44363624962769044No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT2390.18733050116787633No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG2020.15832954492012982No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC1710.1340314464422881No Hit
GTGTAAATACAATAAGTAATGAGGAAACAGGTAACGAATTACAGAAAACC1710.1340314464422881No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT1660.13011239830070073No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT1650.12932858867238325No Hit
CCTAAGATGTGGGAGATGGGAACAACCAAAGAACTGGTGCAAAACACCTA1640.12854477904406578No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT1550.12149049238920852No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT1530.11992287313257355No Hit
GTATTTACACAGTCCCTCCACAACTTCCATAAGAAGTTTTCGTTAGCTTT1460.11443620573435125No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC1400.10973334796444639No Hit
ACATTGTCTCTGGAGACCATATAAACAACAAAGATAGCTATCATCAGTGT1390.10894953833612891No Hit
CCCATACACTGGTGTTCCCCCTTATTCTCATGGAACAGGAACAGGCTACA1320.1034628709379066No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA1300.10189525168127166No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC1290.10111144205295418No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATTG450.0145.05
ACAACAG306.4574124E-10145.01
CGGTGCA351.888111E-9124.285712
GGATTGA600.0108.756
GAGCAGA203.8582433E-4108.751
TTTAAGA352.7478382E-7103.571439
GAAACAC607.2759576E-1296.6666644
CTTTAAG406.0867205E-790.6258
TAGAAAC702.910383E-1182.857142
AGAAGCG300.001931859372.55
GAAGCGG300.001931859372.56
GAGCATC502.294706E-672.54
GTAACAA1550.065.483874
AGCATCT702.3182838E-762.1428575
ACAAGAG1650.061.5151567
AAACACG1007.1122486E-1058.05
GTAGAAA1353.6379788E-1253.7037051
ACGAGCC553.208348E-452.7272764
CGAGCCT553.208348E-452.7272765
AACACGA1004.659887E-850.751
Produced by FastQC (version 0.11.2)